Acetochlor application at field-rate compromises the locomotion of the jumping spider Plexippus paykulli (Araneae: Salticidae)

Acetochlor application at field-rate compromises the locomotion of the jumping spider Plexippus paykulli (Araneae: Salticidae)

Hafiz Muhammad Tahir1*, Tahira Noor1, Muhammad Farooq Bhatti3, Malook Bano1,
Abida Butt2, Imtiaz Alam2, Muhammad Arshad1, Muhammad Khalid Mukhtar1,
Shafaat Yar khan’, Khawaja Raees Ahmed’ and Muhammad Mohsin Ahsan’

1

Department of Biological Sciences, University of Sargodha, Pakistan.

2Department of Zoology, University of the Punjab, Lahore, Pakistan.

“Department of Sericulture, Ravi Road Lahore, Pakistan.

Accepted 10 May, 2012

The present study addresses the direct effects of acetochlor (herbicide) on the mortality, behaviour and growth of the Plexippus paykulli (Savigny and Audouin, 1827). For the study, spider specimens were captured from the ornamental plants of University of Sargodha, Pakistan using the Jerking method. Spiders were exposed to the herbicide treated filter papers. Mortality was very low in the dosed spiders (13%) at the field rate concentration of herbicide. Non-significant difference was recorded in the time spent by tested spiders on the herbicide or water treated part of the filter paper. Locomotory time was significantly reduced in the treated group of spiders compared to group of spiders not exposed to the treated filter papers (control group). Results of the study suggest that acetochlor has negligible effects on the experimental species (P. paykulli) under the laboratory conditions. It is recommended that before considering acetocholr for integrated pest management (IPM), it is necessary to investigate the indirect effects of this herbicide on the experimental species. Furthermore, effects of acetochlor on other spider species and arthropod predator should also be considered.

Key words: Salticidae, integrated pest management (IPM), jumping spiders, Plexippus paykulli

INTRODUCTION

In agro-ecosystems, role of spiders is very important as they limit the growth of insect pests (Snyder and Wise, 1999; Nyffeler, 2000; Sigsgaard, 2000; Maloney et al., 2003; Venturino et al., 2008; Chatterjee et al., 2009). Many researchers have documented the impact of insecticides on survival and behaviour of spiders (Asteraki et al., 1992, Fernandes et al., 2010) but the data on the effects of herbicides on mortality and behaviour is rare.

According to the study of Yardim and Edward (1998), Haughton et al. (2001b) and Michalkova and Pekar (2009), effects of herbicide on the spiders are negligible. However, many researchers claimed that herbicides not

‘Corresponding author. E-mail: hafiztahirpk1@yahoo.com.

only affect the survival of spiders but also influence their behavior (Haughton et al., 2001b; Bell et al., 2002; Tietjen, 2006; Deng et al., 2007; Wilder and Rypstra, 2007). Herbicide may reduce the predatory potential of spiders, as the pests might become distasteful with herbicide application and be avoided by natural enemies. This increases the chance of escape for the prey from predation (Cohn and MacPhail, 1996; Amalin et al., 2001).

The cosmopolitan, Plexippus paykulli (Savigny and Audouin, 1827) is a dominant jumping spider species and active hunter in many agricultural crops and orchards (Skinner, 1974; Muma, 1975; Mansour et al., 1982). The adults and immature can be found throughout the year in different agricultural fields (Edward, 1979).

and India (Rao et al., 1981). This spider is a polyphagous predator feeding on a wide variety of arthropod taxa including Odonata, Orthoptera, Homoptera, Lepidoptera, Diptera, Hymenoptera and other Araneae (Edwards et al., 1974; Muma, 1975; Edwards, 1979; Miah et al., 1986; Jackson and Macnab, 1989). They also significantly control the populations of some important vectors (that is, muscoid flies, mosquitoes etc.) of human and domestic animal diseases (Edward, 1979).

Acetochlor (2-Chloro-N-ethoxymethyl-6’-ethylacet-o- toluidide) is widely used in Pakistan for the control of weeds in various agricultural crops. The present study was designed to investigate the effects of acetochlor on the mortality, growth, and locomotory behaviour of P. paykulli.

MATERIALS AND METHODS

The study was conducted from November 2010 to March 2011 in the Department of Biological Sciences, University of Sargodha, Pakistan. For the experiment, live specimens of spiders were collected from the ornamental plants (Polyalthia longifolia: Annonaceae and Duranta repers: Verbenaceae) of the University of Sargodha.

Spiders were collected by jerking the plants over a white cloth spread on the floor. Collected spiders were individually placed in plastic containers covered with mesh cloth to be taken to the laboratory. Collected spiders were brought to the laboratory and were identified by using Dyal (1935). In the laboratory, each spider was placed individually in the plastic container (8.75 cm wide and 3.75 cm high) containing moist sand at the bottom and fed Drosophilla melanogaster. Containers were covered with mesh cloth and maintained at room temperature (32 to 38°C), humidity (60 to 78%) and photoperiod of 12: 12 h (Light: Dark).

For residual bioassay, only field rate concentrations (recommended rate to spray in the agricultural fields) of acetochlor (200 ml/acre) were used. Filter papers (6 x 12 inches) were dipped in the herbicide solution and dried by placing in the sunlight for one hour. After drying, filter papers were rolled and kept in the glass jars (9 cm long and 3.5 cm wide). Spiders (n = 30, 15 for treatment group and 15 for control group) were placed in these glass jars for one hour to expose them to the herbicide. After exposure, treated spiders were placed individually in the clean glass vials (9 cm long and 3.5 cm wide), covered with mesh cloth.

Status of spiders (active, inactive, dead or paralyzed) was checked in each glass vial after 4, 8, 16, 24, and 48 h intervals. Spiders were considered active if they showed normal locomotory activity, inactive if not showing locomotory activity, paralyzed if there is no movement of body parts but responded when touched with needle and dead if spiders did not respond even when touched with a needle.

To study the avoidance behavior of P. paykulli, Wattman’s filter papers (12 x 9 inches) were cut into two equal halves (by drawing a line in the centre of the filter paper). One half of filter paper was dipped in distilled water (control) while the other half in the field rate concentration of herbicide (acetochlor) solution. Filter papers were dried in the sunlight for one hour. After drying the filter papers, both halves of filter papers were joined together by using scotch tape. Each filter paper was placed in a petri dish (60 mm diameter) and single spider was released in each petri dish (n = 30). Before recording the data, each spider was given 15 min for acclimatization. Time spent by each spider on the herbicide treated or control part of the filter paper was recorded till half an hour. A stop watch was used to record time duration.

To check the locomotory behaviour, spiders (n = 30) were exposed to the filter papers treated with field rate of herbicide or distilled water. The method of preparation of filter paper was same as described in the avoidance behaviour experiment. The time spent in the locomotory or non locomotory activity was recorded till half an hour.

To evaluate the effect of herbicide on the growth of body, immature spiders of same size and weight were selected. Spiders were divided in two groups (that is, treated and control groups). Each group contained 30 specimens. Spiders of both groups were maintained at room temperature (32 to 38°C), humidity (60 to 78%) and a light: dark cycle of 12:12 h. The experimental group was exposed to the herbicide treated filter papers (field rate concentration) for one hour every five days for a one month while control group with distilled water. Experiment was replicated thrice.

To fulfill their nutritional requirements, wet cotton was dipped in a mixture of honey and soya sauce and kept in each glass vial. Each glass vial was covered with mesh cloth and tightened with rubber band. The cotton was replaced each day till 30 day with a same procedure. After 30 days, spider specimens were preserved in the mixture of glycerol (30%) and alcohol (70%).

Kolmogorov-Smirnov test was used to check the normality of the data before using any statistical analysis. A T-test was used to compare the time spend by spiders on the insecticide treated or water treated part of filter paper. A separate T- test was used to compare the body measures of treated spiders with the control group of spiders. Minitab 13.2 was used for the statistical analyses.

RESULTS

Although there was higher mortality in the treated group of spiders group compared to the control group (at recommended field rate) but statistically, difference was not significant (df = 28; t = 1.80; P = 0.08). The activity of the herbicide treated spiders was reduced just after the exposure but with the passage of time (after 8 h of exposure), we observe normal activity in 90% of herbicide treated spiders.

  1. paykulli did not avoid the surface (filter paper) treated with acetochlor as no difference was recorded in the time spent by P. paykulli on the herbicide or water treated part of filter paper (df = 58; t = – 0.88; P = 0.34; Figure 1). Non locomotory time was significantly increased in the dosed spiders compared to the control group of spiders (df = 58; t = 2.36; P = 0.02; Figure 2). There was no significant difference in the total length, carapace length, carapace width and wet weight of the herbicide treated and control spiders (Table 1).

DISCUSSION

Our results of low mortality of spiders with herbicides are in accordance with the findings of many other researchers. Pekar (2002) reported that immature spiders Theridion impressum (L. Koch), under laboratory conditions were not killed by the herbicides metazachlor, clomazone and clopyralid. Similarly, Haughton et al. (2001b) and Benamu et al. (2010) also confirmed that mortality rates of Lepthyphantes tenuis (Blackwall) treated with widely used herbicide glyphosphate are negligible. But many researchers claimed that spider number is significantly affected even by a relatively low rate of herbicide application (Baines et al., 1998; Bell et al., 2002). Some other researchers also reported the lethal effects of herbicides on the terrestrial arthropods including spiders (Lindsay and French, 2004; Cauble and Wagner, 2005; Tietjen, 2006; Michalkova and Pekar, 2009).

Evans et al. (2010) reported that lycosid spider Pardosa milvina (Hentz, 1844) avoided surfaces where herbicides had been applied. But in the present study, P. paykulli did not avoid the surface (filter paper) treated with acetochlor. Pekar and Hadded (2005) reported that herbicide and pesticide residue can be highly repellent to the spiders. But in another study, Michalkova and Pekar (2009) suggested that glyphosate is safe for the lycosid spiders.

We observed increased non locomotory activity in the spiders exposed with herbicide. Evans et al. (2010) also observed in their study that P. milvina moved less when exposed to the herbicide (glyphosphate-based). The increased non locomotory time may have negative impact on spiders as it may affect their colonization rates, foraging success and reproductive rates.

In the field increased non locomotory time may also increase the chance that dosed spiders may spend more time on the herbicide treated patches, which in turn would have negative effects on the survival of the spiders.

Evans et al. (2010) further suggested in their study that speeds of the spiders increased over the substrate treated with the herbicides, which allow the spiders to spend less time on the contaminated substrate and in this way minimize the risk of herbicide exposure. In the present study, we did not study the effect of herbicide on the speed of the spiders.

Our results are in contrast with the findings of Benamu et al. (2010) who reported negative effects of the herbicides on prey consumption, web building, fecundity, fertility and developmental of spiders. Similar results herbicide effects on the development and reproduction have also been reported by Schneider et al. (2009). Our findings are also contradictory to the results of Zheng- Tian et al. (2008) who reported significant influence of Butachlor on the body weight, survival rate and life history of Pirata subpiraticus (Bosenberg and Strand, 1906).

The present study suggested that acetochlor has negligible effect on the mortality of studied species. Locomotory time is significantly reduced in herbicide treated spiders, which may have negative impact on the predatory potential of the studied species. As some researchers have reported negative effects of herbicides, some on prey consumption, fecundity, fertility and developmental of many spider species, it can be predicted that affects of herbicides on the spiders species are species specific.

Although acetochlor appeared safe for the studies species but before the recommendation of acetochlor to be
use in integrated pest management, it is necessary to investigate the effects of this herbicide on other spider species and other arthropod predators.

African Journal of Agricultural Research Vol. 7(22), pp. 3329-3333, 12 June, 2012

Available online at http://www.academicjournals.org/AJAR

DOI: 10.5897/AJAR11.2182

ISSN 1991-637X ©2012 Academic Journals

REFERENCES

Asteraki EJ, Hanks, CB, Clements RO (1992). The impact of the chemical removal of the hedge-base flora on the community structure of carabid beetles (Coleoptera: Carabidae) and spiders (Araneae) of the hedge bottom. J. Appl. Entomol., 113: 398-406.

Amalin DM, Pena JE, Mcsorley R, Browning HW, Crane JH (2001). Comparison of different sampling methods and effect of pesticide application on spider populations in line orchard in South Florida. Environ. Entomol., 30: 1021-1027.

Bell JR, Haughton Aj, Boatman ND, Wilcox A (2002). Do incremental increases of the herbicide glyphosate have indirect consequences for spider communities? J. Arachnol., 30: 288-297.

Benamu MA , Schneider MI , Sanchez, NE (2010). Effects of the herbicide glyphosate on biological attributes of Alpaida veniliae (Araneae, Araneidae), in laboratory. Chemosphere, 78: 871-876.

Cohn J, Macphail RC (1996). Ethological and experimental approaches to behavior analysis: implications for ecotoxicology. Environ. Health Prospect, 104: 299-305.

Chatterjee S, Isaia M, Venturino E (2009). Spiders as biological controllers in the agroecosystem. J. Theoretical Biol., 258: 352-362.

Cauble K, Wagner RS (2005). Sublethal effects of the herbicide glyphosate on amphibian metamorphosis and development. Bull. Environ. Contam. Toxicol., 75: 429-435.

Deng L, Dai J, Cao H, Xu M (2007). Effects of methamidophos on the predating behavior of Hylyphantes graminicola (Sundevall) (Araneae: Linyphiidae). Environmental Toxicol. Chem., 26: 478-482.

Dyal S (1935). Fauna of Lahore: Spiders of Lahore. Bull. Zool. Punjab Univ., 1: 119-252.

Edwards GB, Carroll JF, Whitcomb WH (1974). Stoidis aurata (Araneae: Salticidae), a spider predator of ants. Florida Entomologist, 57: 337-346.

Edwards GB (1979). Pantropical jumping spiders occurring in Florida (Araneae: Salticidae). Florida Dept. Agric. Consum. Serv. Div. Plant Ind. Entomol. Circ., 199. p. 2

Evans SC, Shaw EM, Rypstra Ann L (2010). Exposure to a glyphosate- based herbicide affects agrobiont predatory arthropod behaviour and long-term survival. Ecotoxicology, 19: 1249-1257.

Fernandes FL, Bacci L, Fernandes MS (2010). Impact and Selectivity of Insecticides to Predators and Parasitoids. Entomo Brasilis, 3(1): 01-10.

Haughton AJ, Bell JR, Wilcox A, Boatman ND (2001b). The effect of the herbicide glyphosate on non-target spiders. Part I. Direct effects on Lepthyphantes tenuis under laboratory conditions. Pest Manage. Sci., 57: 1033-1036.

Jackson RR, Macnab A (1989). Display and predatory behaviour of Plexippus paykulli, a jumping spider (Araneae: Salticidae) from Florida. New Zealand J. Zool., 16: 151-168.

Lindsay EA, French K (2004). Chrysanthemoides monilifera ssp. Rotundata invasion alters decomposition rates in coastal areas of South Australia. For. Ecol. Manage., 198: 387-399.

Maloney D, Drummond FA, Alford R (2003). Spider predation in agroecosystems: Can spiders effectively control pest populations? Maine Agric. For. Exp. Station Tech. Bull., pp.190: 32.

Muma MH (1975). Spiders in Florida citrus groves. Florida Entomologist, 58: 83-90.

Mansour F, Ross JW, Edwards GB, Whitcomb WH, Richman DB, 1982. Spiders of Florida citrus groves. Florida Entomologist, 16: 1114-1123.

Miah MA, Hamid AK, Qudrat-E-Khuda M, Shahjahan M (1986). The problems of Pyrilla perpusilla and the impact of its natural enemies. Bangladesh J. Zool., 14: 9-14.

Michalkova V, Pekar S (2009). How glyphosate altered the behavior of agrobiont spiders (Araneae: Lycopsida) and beetles (Coleoptera: Carabidae). Biological Control, 51: 444-449.

Nyffeler M (2000). Ecological impact of spider predation: A critical assessment of Bristowe’s and Turnbull’s estimates. 

Arachnological Soc., 11(9): 367-373.

Pekar S (2002). Susceptibility of the spider Theridion impressum to 17 pesticides. J. Pest Sci., 75: 51-55.

Pekar S, Haddad CR (2005). Can agrobiont spiders (Araneae) avoid a surface with pesticide residues? Pest Manage. Sci., 61: 1179-1185.

Rao PR, Kankka M, Raju A, Appa Rao RV, Moorthy K, Rao BH (1981). Note on a new record of spider predators of Amrasca biguttula biguttula, a serious pest on mesta from Andhra Pradesh, India. Indian J. Agric. Sci., 51: 203-204.

Schneider MI, Sanhez M, Pineda S, Chi H, Ronco A (2009). Impact of glyphosate on the development, fertility and demography of Chrysoperla externa (Neuroptera: Chrysopidae): ecological approach. Chemosphere, 76: 1451-1455.

Skinner RB (1974). The relative and seasonal abundance of spiders from the herb-shrub stratum of cotton fields and the influence of peripheral habitat on spider populations. MS Thesis, Auburn University.

Snyder WE, WISE DH (1999). Predator Interference and the Establishment of Generalist Predator Populations for Biocontrol. Biological Control, 15: 283-292.

Sigsgaard L (2000). Early season natural biological control of insect pests in rice by spiders – and some factors in the management of the cropping system that may affect this control. European Arachnol., pp. 57-64

Tietjen WJ (2006). Pesticides affect the mating behavior of Rabidosa rabida (Araneae, Lycosidae). J. Arachnol., 34: 285-288.

 

Venturino E, Isaia M, Bona F, Chatterjee S, Badino G (2008). Biological controls of intensive agro-ecosystems: wanderer spiders in the Langa Astigiana. Ecological Complexity, 5: 157-164.

Wilder SM, Rypstra. Ann L (2007). Prior encounters with the opposite sex affect male and female mating behavior in a wolf spider (Araneae: Lycopsidae). Behavioral Ecol. Sociol., 62(11): 1814-1820.

Yardim EN, Edwards CA (1998). The influence of chemical management of pests, diseases and weeds on pest and predatory arthropods associated with tomatoes. Agric. Ecosyst. Environ., 70: 31 – 48.

Zheng-Tian Z, Yu P, Zi-An L, Rui-Qing D (2008). The Toxicity and Effects of Herbicide-butachlor on Growth and Development of Spiders in the Rice-field. Chinese J. Zool., DOI: CNKI:SUN:BIRD.0.2008-05-021.

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